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BMC Genomics

, 5:47

First Online: 19 July 2004Received: 02 March 2004Accepted: 19 July 2004


BackgroundTo identify the spectrum of malignant attributes maintained outside the host environment, we have compared global gene expression in primary breast tumors and matched short-term epithelial cultures.

ResultsIn contrast to immortal cell lines, a characteristic -limited proliferation- phenotype was observed, which included over expressed genes associated with the TGFβ signal transduction pathway, such as SPARC, LOXL1, RUNX1, and DAPK1. Underlying this profile was the conspicuous absence of hTERT expression and telomerase activity, a significant increase in TβRII, its cognate ligand, and the CDK inhibitor, p21. Concurrently, tumor tissue and primary cultures displayed low transcript levels of proliferation-related genes, such as, TOP2A, ANKT, RAD51, UBE2C, CENPA, RRM2, and PLK.

ConclusionsOur data demonstrate that commonly used immortal cell lines do not reflect some aspects of tumor biology as closely as primary tumor cell cultures. The gene expression profile of malignant tissue, which is uniquely retained by cells cultured on solid substrates, could facilitate the development and testing of novel molecular targets for breast cancer.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-5-47 contains supplementary material, which is available to authorized users.

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Author: Shanaz H Dairkee - Youngran Ji - Yong Ben - Dan H Moore - Zhenhang Meng - Stefanie S Jeffrey

Source: https://link.springer.com/article/10.1186/1471-2164-5-47

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