Dynamic localization of SPE-9 in sperm: a protein required for sperm-oocyte interactions in Caenorhabditis elegansReport as inadecuate




Dynamic localization of SPE-9 in sperm: a protein required for sperm-oocyte interactions in Caenorhabditis elegans - Download this document for free, or read online. Document in PDF available to download.

BMC Developmental Biology

, 3:10

First Online: 03 December 2003Received: 20 September 2003Accepted: 03 December 2003

Abstract

BackgroundFertilization in Caenorhabditis elegans requires functional SPE-9 protein in sperm. SPE-9 is a transmembrane protein with a predicted extracellular domain that contains ten epidermal growth factor EGF-like motifs. The presence of these EGF-like motifs suggests that SPE-9 is likely to function in gamete adhesive and-or ligand-receptor interactions.

ResultsWe obtained specific antisera directed against different regions of SPE-9 in order to determine its subcellular localization. SPE-9 is segregated to spermatids with a pattern that is consistent with localization to the plasma membrane. During spermiogenesis, SPE-9 becomes localized to spiky projections that coalesce to form a pseudopod. This leads to an accumulation of SPE-9 on the pseudopod of mature sperm.

ConclusionsThe wild type localization patterns of SPE-9 provide further evidence that like the sperm of other species, C. elegans sperm have molecularly mosaic and dynamic regions. SPE-9 is redistributed by what is likely to be a novel mechanism that is very fast ~5 minutes and is coincident with dramatic rearrangements in the major sperm protein cytoskeleton. We conclude that SPE-9 ends up in a location on mature sperm where it can function during fertilization and this localization defines the sperm region required for these interactions.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-213X-3-10 contains supplementary material, which is available to authorized users.

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Author: Sonia Zannoni - Steven W L-Hernault - Andrew W Singson

Source: https://link.springer.com/article/10.1186/1471-213X-3-10







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