IL-13 expression by blood T cells and not eosinophils is increased in asthma compared to non-asthmatic eosinophilic bronchitisReport as inadecuate




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BMC Pulmonary Medicine

, 9:34

First Online: 14 July 2009Received: 02 October 2008Accepted: 14 July 2009

Abstract

BackgroundIn asthma interleukin IL-13 is increased in the airway compared with non-asthmatic eosinophilic bronchitis. Whether this differential expression is specific to the airway or is more generalised is uncertain.

MethodsWe sought to examine IL-13 expression in peripheral blood T-cells and eosinophils in asthma and non-asthmatic eosinophilic bronchitis. Peripheral blood CD3+ cell and eosinophil intracellular IL-13 expression from subjects with asthma, non-asthmatic eosinophilic bronchitis and healthy controls was assessed. The effect of priming by asthmatic serum on the release of IL-13 by peripheral blood mononuclear cells from healthy subjects was examined and the serum from these subjects was analysed for a range of chemokines and cytokines.

ResultsThe median IQR% intracellular IL-13 expression by CD3+ cells was increased in asthma 5.3 2.7–9.8%; n = 12 compared to non-asthmatic eosinophilic bronchitis 1.1 0.5–3%; n = 7 and healthy controls 1.7 0.2–3%; n = 9 p = 0.02, but was not significantly different in eosinophils across the groups. IL-13 released from healthy peripheral blood mononuclear cells n = 10 was increased by asthmatic serum 117 47.8–198pg-ml compared to control 78.5 42.6–128pg-ml; p = 0.02, but was not affected by non-asthmatic serum.

ConclusionOur findings support the view that IL-13 expression is increased in peripheral blood-derived T cells in asthma and that asthmatic serum up-regulates IL-13 release from healthy peripheral blood mononuclear cells.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2466-9-34 contains supplementary material, which is available to authorized users.

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Author: Salman Siddiqui - Glenn Cruse - Susan Mckenna - William Monteiro - Vijay Mistry - Andrew Wardlaw - Christopher Brightling

Source: https://link.springer.com/







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