Proteomic Characterization of Lytic Bacteriophages of Staphylococcus aureus Isolated from Sewage Affluent of IndiaReport as inadecuate




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International Scholarly Research Notices - Volume 2014 2014, Article ID 265298, 6 pages -

Research Article

School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India

Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India

Received 29 March 2014; Accepted 25 June 2014; Published 14 September 2014

Academic Editor: Tzi Bun Ng

Copyright © 2014 Kamalpreet Kaur Sangha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Staphylococcus aureus is a Gram-positive bacterium that causes a variety of diseases, including bovine mastitis, which has severe economic consequences. Standard antibiotic treatment results in selection of resistant strains, leading to need for an alternative treatment such as bacteriophage therapy. Present study describes isolation and characterization of a staphylococcal phage from sewage samples. S. aureus isolates obtained from microbial type culture collection MTCC, Chandigarh, India, were used to screen staphylococcal phages. A phage designated as ΦMSP was isolated from sewage samples by soft agar overlay method. It produced clear plaques on tryptone soya agar overlaid with S. aureus. Transmission electron microscopy revealed that the phage had an icosahedral symmetry. It had 5 major proteins and possessed a peptidoglycan hydrolase corresponding to 70 kDa. ΦMSP infection induced 26 proteins to be uniquely expressed in S. aureus. This phage can be proposed as a candidate phage to treat staphylococcal infections.





Author: Kamalpreet Kaur Sangha, B. V. Sunil Kumar, Ravi Kant Agrawal, Dipak Deka, and Ramneek Verma

Source: https://www.hindawi.com/



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