Biological Assessment of a Calcium Silicate Incorporated Hydroxyapatite-Gelatin Nanocomposite: A Comparison to Decellularized Bone MatrixReport as inadecuate




Biological Assessment of a Calcium Silicate Incorporated Hydroxyapatite-Gelatin Nanocomposite: A Comparison to Decellularized Bone Matrix - Download this document for free, or read online. Document in PDF available to download.

BioMed Research International - Volume 2014 2014, Article ID 837524, 12 pages -

Research Article

NC Oral Health Institute, School of Dentistry, University of North Carolina, CB 7454, Chapel Hill, NC 27599, USA

Department of Diagnostic Sciences, School of Dentistry, University of North Carolina, CB 7454, Chapel Hill, NC 27599, USA

Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455, USA

Department of Orthodontics, School of Dentistry, University of North Carolina, 275 Brauer Hall, CB 7454, Chapel Hill, NC 27599, USA

Received 31 December 2013; Revised 9 March 2014; Accepted 22 May 2014; Published 26 June 2014

Academic Editor: Mitsuo Yamauchi

Copyright © 2014 Dong Joon Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Our laboratory utilized biomimicry to develop a synthetic bone scaffold based on hydroxyapatite-gelatin-calcium silicate HGCS. Here, we evaluated the potential of HGCS scaffold in bone formation in vivo using the rat calvarial critical-sized defect CSD. Twelve Sprague-Dawley rats were randomized to four groups: control defect only, decellularized bone matrix DECBM, and HGCS with and without multipotent adult progenitor cells MAPCs. DECBM was prepared by removing all the cells using SDS and NH4OH. After 12 weeks, the CSD specimens were harvested to evaluate radiographical, histological, and histomorphometrical outcomes. The in vitro osteogenic effects of the materials were studied by focal adhesion, MTS, and alizarin red. Micro-CT analysis indicated that the DECBM and the HGCS scaffold groups developed greater radiopaque areas than the other groups. Bone regeneration, assessed using histological analysis and fluorochrome labeling, was the highest in the HGCS scaffold seeded with MAPCs. The DECBM group showed limited osteoinductivity, causing a gap between the implant and host tissue. The group grafted with HGCS+MAPCs resulting in twice as much new bone formation seems to indicate a role for effective bone regeneration. In conclusion, the novel HGCS scaffold could improve bone regeneration and is a promising carrier for stem cell-mediated bone regeneration.





Author: Dong Joon Lee, Ricardo Padilla, He Zhang, Wei-Shou Hu, and Ching-Chang Ko

Source: https://www.hindawi.com/



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