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Biochemistry Research InternationalVolume 2012 2012, Article ID 691363, 8 pages

Research Article

Biophysical Sciences Institute, Department of Chemistry and School of Biological and Biomedical Sciences, Durham University, Durham DH1 3LE, UK

School of Medicine and Health, Durham University, Queen-s Campus, Stockton-on-Tees TS17 6BH, UK

Received 28 June 2011; Revised 18 July 2011; Accepted 22 July 2011

Academic Editor: Terry K. Smith

Copyright © 2012 Hayder Z. Ali et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Leishmania species are the causative agents of the leishmaniases, a spectrum of neglected tropical diseases. Amastigote stage parasites exist within macrophages and scavenge host factors for survival, for example, Leishmania species utilise host sphingolipid for synthesis of complex sphingolipid. In this study L. mexicana endocytosis was shown to be significantly upregulated in amastigotes, indicating that sphingolipid scavenging may be enhanced. However, inhibition of host sphingolipid biosynthesis had no significant effect on amastigote proliferation within a macrophage cell line. In addition, infection itself did not directly influence host biosynthesis. Notably, in contrast to L. major, L. mexicana amastigotes are indicated to possess a complete biosynthetic pathway suggesting that scavenged sphingolipids may be nonessential for proliferation. This suggested that Old and New World species differ in their interactions with the macrophage host. This will need to be considered when targeting the Leishmania sphingolipid biosynthetic pathway with novel therapeutics.





Author: Hayder Z. Ali, Clare R. Harding, and Paul W. Denny

Source: https://www.hindawi.com/



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