Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivityReport as inadecuate




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1 Immunité et cancer 2 Laboratoire MSC - Laboratoire Matière et Systèmes Complexes 3 UFR 925 - Faculté de Physique UPMC 4 IC - Institut Cochin

Abstract : T cells are mechanosensitive but the effect of stiffness on their functions is still debated. We characterize herein how human primary CD4 + T cell functions are affected by stiffness within the physiological Young-s modulus range of 0.5 kPa to 100 kPa. Stiffness modulates T lymphocyte migration and morphological changes induced by TCR-CD3 triggering. Stiffness also increases TCR-induced immune system, metabolism and cell-cycle-related genes. Yet, upon TCR- CD3 stimulation, while cytokine production increases within a wide range of stiffness, from hundreds of Pa to hundreds of kPa, T cell metabolic properties and cell cycle progression are only increased by the highest stiffness tested 100 kPa. Finally, mechanical properties of adherent antigen-presenting cells modulate cytokine production by T cells. Together, these results reveal that T cells discriminate between the wide range of stiffness values found in the body and adapt their responses accordingly.





Author: Michael Saitakis - Stéphanie Dogniaux - Christel Goudot - Nathalie Bufi - Sophie Asnacios - Mathieu Maurin - Clotilde Randriamam

Source: https://hal.archives-ouvertes.fr/



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