Follow-Up of Stable Chromosomal Aberrations in Gamma-Ray Irradiated Non-human PrimatesReport as inadecuate




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* Corresponding author 1 DRPH-SRBE-LDB - Laboratoire de dosimétrie biologique 2 DRPH-SRBE-LTCRA - Laboratoire de Thérapie Cellulaire et de Radioprotection Accidentelle 3 DRPH-SRBE - Radiobiologie et épidémiologie

Abstract : Purpose: The purpose of this study was to examine a new approach to retrospective biological dosimetry, by using a long-term animal model to determine the stability of translocation frequency after in vivo irradiation. While the frequency of dicentrics is known to decrease over time, the persistence of more stable chromosomal aberrations such as translocations could be useful if their stability were definitively proved. Materials and Methods: Four monkeys Macaca fascicularis were exposed to two different doses of ionising radiation: 2 Gy whole body irradiation for two and 4 Gy for two others. Blood samples were obtained at various times after irradiation. Both total and two-way translocations were detected by fluorescence in situ hybridisation. Translocations were scored in stable cells, that is, those without dicentrics, rings or fragments. The course of translocation frequency was analysed at four time-points: one hour H1, 2 months M2, 10 months M10 and 31 months M31 after irradiation. Results: We observed two separate trends in translocation frequency: total translocation frequency decreased slightly in animals irradiated with a dose of 2 Gy, while two-way translocation frequency was relatively stable in all irradiated animals. Conclusions: We confirmed the long-term stability of translocations and found that it seems to depend on the type of the translocation recorded. Overall translocations were stable for up to 31 months regardless of dose, but two-way translocations were more stable than those that were non-reciprocal, especially in stable cells.





Author: Eric Gregoire - Irène Sorokine-Durm - Jean-Marc Bertho - N. Jacquet - Martine Delbos - Christelle Demarquay - Philippe Voisin -

Source: https://hal.archives-ouvertes.fr/



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