Reversal of Ampicillin Resistance in MRSA via Inhibition of Penicillin-Binding Protein 2a by Acalypha wilkesianaReport as inadecuate




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BioMed Research InternationalVolume 2014 2014, Article ID 965348, 7 pages

Research ArticleFaculty of Science, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor, Malaysia

Received 26 February 2014; Revised 4 June 2014; Accepted 18 June 2014; Published 30 June 2014

Academic Editor: Ranadhir Chakraborty

Copyright © 2014 Carolina Santiago et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The inhibitory activity of a semipure fraction from the plant, Acalypha wilkesiana assigned as 9EA-FC-B, alone and in combination with ampicillin, was studied against methicillin-resistant Staphylococcus aureus MRSA. In addition, effects of the combination treatment on PBP2a expression were investigated. Microdilution assay was used to determine the minimal inhibitory concentrations MIC. Synergistic effects of 9EA-FC-B with ampicillin were determined using the fractional inhibitory concentration FIC index and kinetic growth curve assay. Western blot experiments were carried out to study the PBP2a expression in treated MRSA cultures. The results showed a synergistic effect between ampicillin and 9EA-FC-B treatment with the lowest FIC index of 0.19 synergism 0.5. The presence of 9EA-FC-B reduced the MIC of ampicillin from 50 to 1.56 μg mL

. When ampicillin and 9EA-FC-B were combined at subinhibitory level, the kinetic growth curves were suppressed. The antibacterial effect of 9EA-FC-B and ampicillin was shown to be synergistic. The synergism is due the ability of 9EA-FC-B to suppress the activity of PBP2a, thus restoring the susceptibility of MRSA to ampicillin. Corilagin was postulated to be the constituent responsible for the synergistic activity showed by 9EA-FC-B.





Author: Carolina Santiago, Ee Leen Pang, Kuan-Hon Lim, Hwei-San Loh, and Kang Nee Ting

Source: https://www.hindawi.com/



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