Novel HPLC Analysis of Hydrocortisone in Conventional and Controlled-Release Pharmaceutical PreparationsReport as inadecuate




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Journal of Pharmaceutics - Volume 2017 2017, Article ID 9495732, 8 pages - https:-doi.org-10.1155-2017-9495732

Research Article

Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology KNUST, Kumasi, Ghana

Department of Pharmaceutics and Microbiology, School of Pharmacy, University of Ghana, Legon, Accra, Ghana

Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology KNUST, Kumasi, Ghana

Correspondence should be addressed to Kwabena Ofori-Kwakye

Received 7 March 2017; Accepted 26 April 2017; Published 4 June 2017

Academic Editor: Nerea Ferreirós Bouzas

Copyright © 2017 Ofosua Adi-Dako et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An isocratic sensitive and precise reverse phase high-performance liquid chromatography RP-HPLC method was developed and validated for the determination and quantification of hydrocortisone in controlled-release and conventional tablets and injections pharmaceutical preparations. Chromatographic separation was achieved on an ODS C18, 5 m, 4.6 × 150 mm, with an isocratic elution using a freshly prepared mobile phase of composition methanol : water : acetic acid 60 : 30 : 10, v-v-v at a flow rate of 1.0 ml-min. The detection of the drug was successfully achieved at a wavelength of 254 nm. The retention time obtained for the drug was 2.26 min. The proposed method produced linear detectable responses in the concentration range of 0.02 to 0.4 mg-ml of hydrocortisone. High recoveries of 98–101% were attained at concentration levels of 80%, 100%, and 120%. The intraday and interday precision RSD were 0.19–0.55% and 0.33–0.71%, respectively. A comparison of hydrocortisone analyses data from the developed method and the official USP method showed no significant difference at a 95% confidence interval. The method was successfully applied to the determination and quantification of hydrocortisone in six controlled-release and fifteen conventional release pharmaceutical preparations.





Author: Ofosua Adi-Dako, Samuel Oppong Bekoe, Kwabena Ofori-Kwakye, Enoch Appiah, and Paul Peprah

Source: https://www.hindawi.com/



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