Synthesis of Water-Soluble Iridium III-Containing Nanoparticles for Biological ApplicationsReport as inadecuate




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Journal of Chemistry - Volume 2015 2015, Article ID 475602, 7 pages -

Research Article

Key Laboratory for Organic Electronics and Information Displays KLOEID and Institute of Advanced Materials IAM, Nanjing University of Posts and Telecommunications NUPT, Nanjing 210046, China

Jiangsu-Singapore Joint Research Center on Organic-Bio-Electronics and Information Displays and Institute of Advanced Materials, Nanjing Tech University, Nanjing 211816, China

Received 27 August 2015; Accepted 20 October 2015

Academic Editor: Jin Geng

Copyright © 2015 Huanzhi Hou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Water-soluble nanoparticles Ir-PGlc-NP, Ir-β-1,3-glucan-NP based on water-soluble glycopolymers PGlc, β-1,3-glucan polysaccharide, and conjugated phosphorescent Ir III complexes were successfully synthesized by self-assembly. The obtained nanoparticles have good spherical morphological characterization with a mean diameter of 50 nm measured by TEM. Ir-PGlc-NP and Ir-β-1,3-glucan-NP showed the same emission maxima at 565 nm in aqueous solution and both caused effective apoptosis and death of HepG2 and Hela cells after being irradiated at 445 nm for 30 min in vitro. Fluorescence cellular imaging was conducted by confocal laser scanning microscopy CLSM using HepG2 cells as the model cell in which the nanoparticles had successfully entered into the cytoplasm with high brightness. Furthermore, after injecting the nanoparticles into live mice in vivo, the real-time fluorescence imaging as well as the nanoparticles distribution in organs at 24 hours after administration indicated that these nanoparticles can serve as fluorescent imaging contrast for further biological applications.





Author: Huanzhi Hou, Pengfei Sun, Quli Fan, Xiaomei Lu, Cheng Xue, Yixue Zhang, Sichao Tian, and Wei Huang

Source: https://www.hindawi.com/



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