Herpesvirus-Mediated Delivery of a Genetically Encoded Fluorescent Sensor to Canine CardiomyocytesReport as inadecuate




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Journal of Biomedicine and BiotechnologyVolume 2009 2009, Article ID 361795, 12 pages

Research Article

Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, H-6720 Szeged, Hungary

Division of Cardiovascular Pharmacology, Hungarian Academy of Sciences, H-6720 Szeged, Hungary

Department of Medical Biology, Faculty of Medicine, University of Szeged, H-6720 Szeged, Hungary

Received 27 February 2009; Accepted 4 May 2009

Academic Editor: Mohamed Boutjdir

Copyright © 2009 János Prorok et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We report the development and application of a pseudorabies virus-based system for delivery of troponeon, a fluorescent sensor to adult canine cardiomyocytes. The efficacy of transduction was assessed by calculating the ratio of fluorescently labelled and nonlabelled cells in cell culture. Interaction of the virus vector with electrophysiological properties of cardiomyocytes was evaluated by the analysis of transient outward current , kinetics of the intracellular transients, and cell shortening. Functionality of transferred troponeon was verified by FRET analysis. We demonstrated that the transfer efficiency of troponeon to cultured adult cardiac myocytes was virtually 100%. We showed that even after four days neither the amplitude nor the kinetics of the current was significantly changed and no major shifts occurred in parameters of transients. Furthermore, we demonstrated that infection of cardiomyocytes with the virus did not affect the morphology, viability, and physiological attributes of cells.





Author: János Prorok, Péter P. Kovács, Attila A. Kristóf, Norbert Nagy, Dóra Tombácz, Judit S. Tóth, Balázs Ördög, Norber

Source: https://www.hindawi.com/



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