Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudatesReport as inadecuate




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BMC Microbiology

, 12:116

First Online: 21 June 2012Received: 01 April 2012Accepted: 31 May 2012DOI: 10.1186-1471-2180-12-116

Cite this article as: Fan, B., Carvalhais, L.C., Becker, A. et al. BMC Microbiol 2012 12: 116. doi:10.1186-1471-2180-12-116

Abstract

BackgroundPlant root exudates have been shown to play an important role in mediating interactions between plant growth-promoting rhizobacteria PGPR and their host plants. Most investigations were performed on Gram-negative rhizobacteria, while much less is known about Gram-positive rhizobacteria. To elucidate early responses of PGPR to root exudates, we investigated changes in the transcriptome of a Gram-positive PGPR to plant root exudates.

ResultsBacillus amyloliquefaciens FZB42 is a well-studied Gram-positive PGPR. To obtain a comprehensive overview of FZB42 gene expression in response to maize root exudates, microarray experiments were performed. A total of 302 genes representing 8.2% of the FZB42 transcriptome showed significantly altered expression levels in the presence of root exudates. The majority of the genes 261 was up-regulated after incubation of FZB42 with root exudates, whereas only 41 genes were down-regulated. Several groups of the genes which were strongly induced by the root exudates are involved in metabolic pathways relating to nutrient utilization, bacterial chemotaxis and motility, and non-ribosomal synthesis of antimicrobial peptides and polyketides.

ConclusionsHere we present a transcriptome analysis of the root-colonizing bacterium Bacillus amyloliquefaciens FZB42 in response to maize root exudates. The 302 genes identified as being differentially transcribed are proposed to be involved in interactions of Gram-positive bacteria with plants.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-12-116 contains supplementary material, which is available to authorized users.

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Author: Ben Fan - Lilia C Carvalhais - Anke Becker - Dmitri Fedoseyenko - Nicolaus von Wirén - Rainer Borriss

Source: https://link.springer.com/



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