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BMC Evolutionary Biology

, 12:144

Genome evolution and evolutionary systems biology

Abstract

BackgroundThe highly improved cognitive function is the most significant change in human evolutionary history.
Recently, several large-scale studies reported the evolutionary roles of DNA methylation; however, the role of DNA methylation on brain evolution is largely unknown.

ResultsTo test if DNA methylation has contributed to the evolution of human brain, with the use of MeDIP-Chip and SEQUENOM MassARRAY, we conducted a genome-wide analysis to identify differentially methylated regions DMRs in the brain between humans and rhesus macaques.
We first identified a total of 150 candidate DMRs by the MeDIP-Chip method, among which 4 DMRs were confirmed by the MassARRAY analysis.
All 4 DMRs are within or close to the CpG islands, and a MIR3 repeat element was identified in one DMR, but no repeat sequence was observed in the other 3 DMRs.
For the 4 DMR genes, their proteins tend to be conserved and two genes have neural related functions.
Bisulfite sequencing and phylogenetic comparison among human, chimpanzee, rhesus macaque and rat suggested several regions of lineage specific DNA methylation, including a human specific hypomethylated region in the promoter of K6IRS2 gene.

ConclusionsOur study provides a new angle of studying human brain evolution and understanding the evolutionary role of DNA methylation in the central nervous system.
The results suggest that the patterns of DNA methylation in the brain are in general similar between humans and non-human primates, and only a few DMRs were identified.

KeywordsDNA methylation Brain evolution Primates Electronic supplementary materialThe online version of this article doi:10.1186-1471-2148-12-144 contains supplementary material, which is available to authorized users.

Jinkai Wang, Xiangyu Cao contributed equally to this work.

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Author: Jinkai Wang - Xiangyu Cao - Yanfeng Zhang - Bing Su

Source: https://link.springer.com/



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