Quantitative trait locus mapping of genes associated with vacuolation in the adrenal X-zone of the DDD-Sgn inbred mouseReport as inadecuate




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BMC Genetics

, 13:95

Complex traits and quantitative genetics

Abstract

BackgroundAdrenal gland of mice contains a transient zone between the adrenal cortex and the adrenal medulla: the X-zone. There are clear strain differences in terms of X-zone morphology. Nulliparous females of the inbred mouse DDD strain develop adrenal X-zones containing exclusively vacuolated cells, whereas females of the inbred mouse B6 strain develop X-zones containing only non-vacuolated cells. The X-zone vacuolation is a physiologic process associated with the X-zone degeneration and is tightly regulated by genetic factors. Identification of the genetic factors controlling such strain differences should help analyze the X-zone function. In this study, a quantitative trait locus QTL analysis for the extent of X-zone vacuolation was performed for two types of F2 female mice: F2A mice F2 mice with the A allele and F2 non-A mice F2 mice without the A allele. These were produced by crossing B6 females and DDD.Cg-A males. DDD.Cg-A is a congenic mouse strain for the A allele at the agouti locus and is used for this study because a close association between the X-zone morphology and the agouti locus genotype has been suggested. The A allele is dominant and homozygous lethal; therefore, living A mice are invariably heterozygotes.

ResultsSingle QTL scans identified significant QTLs on chromosomes 1, 2, 6, and X for F2 non-A mice, and on chromosomes 2, 6, and 12 for F2A mice. The QTL on chromosome 2 was considered to be because of the agouti locus, which has been suggested to be associated with X-zone vacuolation. A significant QTL that interacted with the agouti locus was identified on chromosome 8.

ConclusionsThe extent of X-zone vacuolation in DDD females was controlled by multiple genes with complex interactions. The murine X-zone is considered analogous structure to the human fetal zone. Therefore, the results of this study will aid in understanding function of not only of the X-zone but also of the human fetal zone. Identifying the genes responsible for the QTLs will be essential for understanding the molecular basis of X-zone function, which is currently unclear.

KeywordsAdrenal X-zoneDDD miceQuantitative trait locus QTL mappingElectronic supplementary materialThe online version of this article doi:10.1186-1471-2156-13-95 contains supplementary material, which is available to authorized users.

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Author: Jun-ichiSuto

Source: https://link.springer.com/







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