Myocardial infarct size quantification in mice by SPECT using a novel algorithm independent of a normal perfusion databaseReport as inadecuate




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EJNMMI Research

, 2:64

First Online: 28 December 2012Received: 24 August 2012Accepted: 16 December 2012DOI: 10.1186-2191-219X-2-64

Cite this article as: Roelants, V., Delgaudine, M., Walrand, S. et al. EJNMMI Res 2012 2: 64. doi:10.1186-2191-219X-2-64

Abstract

BackgroundThere is a growing interest in developing non-invasive imaging techniques permitting infarct size IS measurements in mice. The aim of this study was to validate the high-resolution rodent Linoview single photon emission computed tomography SPECT system for non-invasive measurements of IS in mice by using a novel algorithm independent of a normal database, in comparison with histology.

MethodsEleven mice underwent a left coronary artery ligature. Seven days later, animals were imaged on the SPECT 2h30 after injection of 173 27 MBq of Tc-99m-sestamibi. Mice were subsequently killed, and their hearts were excised for IS determination with triphenyltetrazolium chloride TTC staining. SPECT images were reconstructed using the expectation maximization maximum likelihood algorithm, and the IS was calculated using a novel algorithm applied on the 20-segment polar map provided by the commercially available QPS software Cedars-Sinai Medical Center, CA, USA. This original method is attractive by the fact that it does not require the implementation of a normal perfusion database.

ResultsReconstructed images allowed a clear delineation of the left ventricles borders in all mice. No significant difference was found between mean IS determined by SPECT and by TTC staining 37.9 17.5% vs 35.6 17.2%, respectively P = 0.10. Linear regression analysis showed an excellent correlation between IS measured on the SPECT images and IS obtained with TTC staining y = 0.95x + 0.03 r = 0.97; P < 0.0001, without bias, as demonstrated by the Bland-Altman plot.

ConclusionOur results demonstrate the accuracy of the method for the measurement of myocardial IS in mice with the Linoview SPECT system.

KeywordsInfarct sizeQuantificationMouseSPECTElectronic supplementary materialThe online version of this article doi:10.1186-2191-219X-2-64 contains supplementary material, which is available to authorized users.

Vronique Roelants, Marie Delgaudine contributed equally to this work.

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Author: VroniqueRoelants - MarieDelgaudine - StephanWalrand - RenaudLhommel - YvesBeguin - FranoisJamar - Jean-LouisVanoverschelde

Source: https://link.springer.com/







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