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BMC Genomics

, 14:126

Prokaryote microbial genomics

Abstract

BackgroundStaphylococcus aureus is a widely distributed human pathogen capable of infecting almost every ecological niche of the host. As a result, it is responsible for causing many different diseases. S. aureus has a vast array of virulence determinants whose expression is modulated by an intricate regulatory network, where transcriptional factors TFs are the primary elements. In this work, using diverse sequence analysis, we evaluated the repertoire of TFs and sigma factors in the community-associated methicillin resistant S. aureus CA-MRSA strain USA300-FPR3757.

ResultsA total of 135 TFs and sigma factors were identified and classified into 36 regulatory families. From these around 43% have been experimentally characterized to date, which demonstrates the significant work still at hand to unravel the regulatory network in place for this important pathogen. A comparison of the TF repertoire of S. aureus against 1209 sequenced bacterial genomes was carried out allowing us to identify a core set of orthologous TFs for the Staphylococacceae, and also allowing us to assign potential functions to previously uncharacterized TFs. Finally, the USA300 TFs were compared to those in eleven other S. aureus strains including: Newman, COL, JH1, JH9, MW2, Mu3, Mu50, N315, RF122, MRSA252 and MSSA476. We identify conserved TFs among these strains and suggest possible regulatory interactions.

ConclusionsThe analysis presented herein highlights the complexity of regulatory networks in S. aureus strains, identifies key conserved TFs among the Staphylococacceae, and offers unique insights into several as yet uncharacterized TFs.

KeywordsTranscriptional regulators Virulence Gene evolution Staphylococacceae Firmicutes Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-14-126 contains supplementary material, which is available to authorized users.

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Author: Jose Antonio Ibarra - Ernesto Pérez-Rueda - Ronan K Carroll - Lindsey N Shaw

Source: https://link.springer.com/







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