Comparative study of the promotion of porcine fetal fibroblast proliferation by overexpression of two transcriptional variants of SIRT6Report as inadecuate




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Chinese Science Bulletin

, Volume 58, Issue 10, pp 1169–1174

First Online: 23 March 2013Received: 21 May 2012Accepted: 28 September 2012DOI: 10.1007-s11434-013-5706-5

Cite this article as: Xie, X., He, K., Zhang, A. et al. Chin. Sci. Bull. 2013 58: 1169. doi:10.1007-s11434-013-5706-5

Abstract

Mammalian Sirtuin 6 SIRT6 plays an important role in the transcriptional silencing of silent mating-type loci, telomeres, and rDNA. However, the roles of porcine SIRT6 in cell proliferation are poorly understood, and a better knowledge of these will help improve our understanding of the biological mechanisms of cell growth and development. In this study, a novel variant of porcine SIRT6 SIRT6 V2 identified by reverse transcription-polymerase chain reaction and BLAST analysis showed a 124-bp deletion compared to wild-type SIRT6 mRNA SIRT6 V1. Two recombinant plasmids overexpressing SIRT6 V1 and SIRT6 V2 were produced and their roles in the proliferation of porcine fetal fibroblasts PFFs were compared. Cells transfected with SIRT6 V1 proliferated significantly faster than those transfected with SIRT6 V2 P<0.01, though both proliferated significantly faster than cells expressing an empty vector P<0.01. These results might be caused by altered proportions of α helices and β sheets in the SIRT6 V2 structure relative to SIRT6 V1. These results indicate that overexpression of SIRT6 V1-V2 was involved in promoting PFF proliferation. Deletion of a 124-bp sequence attenuated the effects of SIRT6 on cell proliferation, possibly as a result of changes in the proportions of α helices and β sheets in the protein secondary structure.

KeywordsSIRT6 variant overexpression porcine fetal fibroblast proliferation prediction assay This article is published with open access at Springerlink.com

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Author: XiaoXian Xie - Kan He - AiLing Zhang - Hao Zhang - QiShan Wang - JiaQi Li - YuFang Ma

Source: https://link.springer.com/







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