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Radiation Oncology

, 8:141

First Online: 11 June 2013Received: 27 February 2013Accepted: 30 May 2013DOI: 10.1186-1748-717X-8-141

Cite this article as: Niklas, M., Greilich, S., Melzig, C. et al. Radiat Oncol 2013 8: 141. doi:10.1186-1748-717X-8-141

Abstract

BackgroundThe lack of sensitive biocompatible particle track detectors has so far limited parallel detection of physical energy deposition and biological response. Fluorescent nuclear track detectors FNTDs based on Al2O3:C,Mg single crystals combined with confocal laser scanning microscopy CLSM provide 3D information on ion tracks with a resolution limited by light diffraction. Here we report the development of next generation cell-fluorescent ion track hybrid detectors Cell-Fit-HD.

MethodsThe biocompatibility of FNTDs was tested using six different cell lines, i.e. human non-small cell lung carcinoma A549, glioblastoma U87, androgen independent prostate cancer PC3, epidermoid cancer A431 and murine VmDk glioma SMA-560. To evaluate cell adherence, viability and conformal coverage of the crystals different seeding densities and alternative coating with extracellular matrix fibronectin was tested. Carbon irradiation was performed in Bragg peak initial 270.55 MeV u. A series of cell compartment specific fluorescence stains including nuclear HOECHST, membrane Glut-1, cytoplasm Calcein AM, CM-DiI were tested on Cell-Fit-HDs and a single CLSM was employed to co-detect the physical crystal as well as the biological cell layer information.

ResultsThe FNTD provides a biocompatible surface. Among the cells tested, A549 cells formed the most uniform, viable, tightly packed epithelial like monolayer. The ion track information was not compromised in Cell-Fit-HD as compared to the FNTD alone. Neither cell coating and culturing, nor additional staining procedures affected the properties of the FNTD surface to detect ion tracks. Standard immunofluorescence and live staining procedures could be employed to co-register cell biology and ion track information.

ConclusionsThe Cell-Fit-Hybrid Detector system is a promising platform for a multitude of studies linking biological response to energy deposition at high level of optical microscopy resolution.

Electronic supplementary materialThe online version of this article doi:10.1186-1748-717X-8-141 contains supplementary material, which is available to authorized users.

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Author: Martin Niklas - Steffen Greilich - Claudius Melzig - Mark S Akselrod - Jürgen Debus - Oliver Jäkel - Amir Abdollahi

Source: https://link.springer.com/







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