Alterations in expression of endometrial genes coding for proteins secreted into the uterine lumen during conceptus elongation in cattleReport as inadecuate




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BMC Genomics

, 14:321

Non-human and non-rodent vertebrate genomics

Abstract

BackgroundWe hypothesized that genes that are up-regulated in the uterine endometrium at the initiation of conceptus elongation in cattle, and that encode for secreted proteins, contribute to the composition of the uterine luminal fluid ULF and ultimately, drive conceptus elongation. The aims of this study were to: 1 screen endometrial transcriptomic data for genes that encode secreted proteins on Day 13; 2 determine temporal changes in the expression of these genes during the estrous cycle-early pregnancy; 3 determine if expression of these genes is affected by altered concentrations of progesterone P4 in vivo and 4 determine if the protein products of these genes are detectable in ULF.

ResultsOf the fourteen candidate genes examined, quantitative real-time PCR analysis revealed the expression of APOA1, ARSA, DCN, LCAT, MUC13, NCDN, NMN, NPNT, NXPH3, PENK, PLIN2 and TINAGL1 was modulated in the endometrium P<0.05 as the estrous cycle-early pregnancy progressed. APOA1, DCN and NPNT expression was higher in cyclic compared to pregnant heifers, and pregnancy increased P<0.05 the expression of LCAT, NCDN, NMN, PLIN2 and TINAGL1. The magnitude of the increase in expression of APOA1, PENK and TINAGL1 on Day 13 was reduced P<0.05 in heifers with low P4. Furthermore, low P4 decreased P<0.05 the expression of LCAT and NPNT on Day 7, while an early increase P<0.05 in the expression of NXPH3 and PLIN2 was observed in heifers with high P4. The protein products of 5 of the candidate genes APOA1, ARSA, LCAT, NCDN and PLIN were detected in the ULF on either Days 13, 16 or 19 of pregnancy.

ConclusionUsing a candidate gene approach, we determined that both P4 concentration and the presence of the conceptus alter endometrial expression of PLIN2, TINAGL1, NPNT, LCAT, NMN and APOA1. Comparison of the expression profiles of these genes to proteins detected in ULF during conceptus elongation i.e., Days 13 through 19 revealed the presence of APOA1, ARSA, LCAT, NCDN as well as members of the PLIN family of proteins that may play roles in driving conceptus elongation in cattle.

KeywordsUterus Endometrium Gene expression Histotroph Embryo Progesterone Estrous cycle Pregnancy Proteomics Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-14-321 contains supplementary material, which is available to authorized users.

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Author: Niamh Forde - Jai P Mehta - Paul A McGettigan - Solomon Mamo - Fuller W Bazer - Thomas E Spencer - Pat Lonergan

Source: https://link.springer.com/







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