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Journal of Hematology and Oncology

, 6:84

First Online: 05 November 2013Received: 25 September 2013Accepted: 29 October 2013DOI: 10.1186-1756-8722-6-84

Cite this article as: Liao, W., Aguila, J.R., Yao, Y. et al. J Hematol Oncol 2013 6: 84. doi:10.1186-1756-8722-6-84

Abstract

Hematopoietic stem cells HSCs are widely used in transplantation therapy to treat a variety of blood diseases. The success of hematopoietic recovery is of high importance and closely related to the patient’s morbidity and mortality after Hematopoietic stem cell transplantation HSCT. We have previously shown that SALL4 is a potent stimulator for the expansion of human hematopoietic stem-progenitor cells in vitro. In these studies, we demonstrated that systemic administration with TAT-SALL4B resulted in expediting auto-reconstitution and inducing a 30-fold expansion of endogenous HSCs-HPCs in mice exposed to a high dose of irradiation. Most importantly, TAT-SALL4B treatment markedly prevented death in mice receiving lethal irradiation. Our studies also showed that TAT-SALL4B treatment was able to enhance both the short-term and long-term engraftment of human cord blood CB cells in NOD-SCID mice and the mechanism was likely related to the in vivo expansion of donor cells in a recipient. This robust expansion was required for the association of SALL4B with DNA methyltransferase complex, an epigenetic regulator critical in maintaining HSC pools and in normal lineage progression. Our results may provide a useful strategy to enhance hematopoietic recovery and reconstitution in cord blood transplantation with a recombinant TAT-SALL4B fusion protein.

KeywordsHematopoietic stem cell Regeneration Bone marrow transplantation SALL4 Recombinant protein Electronic supplementary materialThe online version of this article doi:10.1186-1756-8722-6-84 contains supplementary material, which is available to authorized users.

Wenbin Liao, Jerell R Aguila contributed equally to this work.

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Author: Wenbin Liao - Jerell R Aguila - Yixin Yao - Jianchang Yang - Gary Zieve - Yongping Jiang - Cecilia Avila - Lisa Senzel -

Source: https://link.springer.com/



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