Effect of Carnosine in Experimental Arthritis and on Primary Culture ChondrocytesReport as inadecuate




Effect of Carnosine in Experimental Arthritis and on Primary Culture Chondrocytes - Download this document for free, or read online. Document in PDF available to download.

Oxidative Medicine and Cellular Longevity - Volume 2016 2016, Article ID 8470589, 11 pages -

Research Article

Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dubravska cesta 9, 841 04 Bratislava, Slovakia

Faculty of Medicine, Comenius University in Bratislava, Spitalska 24, 813 72 Bratislava, Slovakia

Faculty of Pharmacy, Comenius University in Bratislava, Odbojarov 10, 832 32 Bratislava, Slovakia

Research Center of Neurology, RAMS, Volokolamskoyeshosse 80, Moscow 125367, Russia

Received 25 September 2015; Revised 27 November 2015; Accepted 8 December 2015

Academic Editor: Victor M. Victor

Copyright © 2016 S. Ponist et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Carnosine’s CARN anti-inflammatory potential in autoimmune diseases has been but scarcely investigated as yet. The aim of this study was to evaluate the therapeutic potential of CARN in rat adjuvant arthritis, in the model of carrageenan induced hind paw edema CARA, and also in primary culture of chondrocytes under H2O2 injury. The experiments were done on healthy animals, arthritic animals, and arthritic animals with oral administration of CARN in a daily dose of 150 mg-kg b.w. during 28 days as well as animals with CARA treated by a single administration of CARN in the same dose. CARN beneficially affected hind paw volume and changes in body weight on day 14 and reduced hind paw swelling in CARA. Markers of oxidative stress in plasma and brain malondialdehyde, 4-hydroxynonenal, protein carbonyls, and lag time of lipid peroxidation and also activity of gamma-glutamyltransferase were significantly corrected by CARN. CARN also reduced IL-1alpha in plasma. Suppression of intracellular oxidant levels was also observed in chondrocytes pretreated with CARN. Our results obtained on two animal models showed that CARN has systemic anti-inflammatory activity and protected rat brain and chondrocytes from oxidative stress. This finding suggests that CARN might be beneficial for treatment of arthritic diseases.

We dedicate this paper to the memory of Professor Alexander A. Boldyrev with respect and gratitude.





Author: S. Ponist, F. Drafi, V. Kuncirova, D. Mihalova, L. Rackova, L. Danisovic, O. Ondrejickova, I. Tumova, O. Trunova, T. Fedo

Source: https://www.hindawi.com/



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