Biological and chemical diversity of cytotoxin-producing symbiotic marine fungi in intertidal zone of DalianReport as inadecuate




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Chinese Science Bulletin

, Volume 58, Issue 19, pp 2290–2297

First Online: 15 September 2012Received: 02 May 2012Accepted: 23 July 2012DOI: 10.1007-s11434-012-5442-2

Cite this article as: Zhang, Y., Mu, J., Feng, Y. et al. Chin. Sci. Bull. 2013 58: 2290. doi:10.1007-s11434-012-5442-2

Abstract

In this study, the biological and chemical diversity of 8 symbiotic marine fungal strains, with strong cytotoxicity against brine shrimp larvae, were investigated by nucleotide sequencing, morphology and cluster analysis of HPTLC fingerprint. These strains were identified by ITS rDNA sequencing, phylogenic analysis, and morphology to be Hypocrea lixii, Chaetomium globosum, Aspergillus fumigatus, Asp. clavatus and Alternaria sp. Their differences in secondary metabolites were shown by cluster analysis of digitalized colors of HPTLC spots, a newly developed method, which produced a similar dendrogram with that of ITS cluster analysis. Furthermore, this method can fully display intraspecific differences and even the remarkable difference in Aspergillus strains which goes beyond the boundary between genera. Their biological-chemical diversity may be the basis of their potent cytotoxicity and implies their potential in producing diversified antitumor or pesticidal constituents.

Keywordssymbiotic marine fungi cytotoxicity diversity rDNA sequencing morphology fingerprint This article is published with open access at Springerlink.com

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Author: Yi Zhang - Jun Mu - Yan Feng - HeNan Li - XueWei Dong

Source: https://link.springer.com/







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