Coordination of glycerol utilization and clavulanic acid biosynthesis to improve clavulanic acid production in Streptomyces clavuligerusReport as inadecuate




Coordination of glycerol utilization and clavulanic acid biosynthesis to improve clavulanic acid production in Streptomyces clavuligerus - Download this document for free, or read online. Document in PDF available to download.

Science China Life Sciences

, Volume 56, Issue 7, pp 591–600

First Online: 06 July 2013Received: 26 April 2013Accepted: 28 May 2013DOI: 10.1007-s11427-013-4507-z

Cite this article as: Guo, D., Zhao, Y. & Yang, K. Sci. China Life Sci. 2013 56: 591. doi:10.1007-s11427-013-4507-z

Abstract

The glycerol utilization gyl operon is involved in clavulanic acid CA production by Streptomyces clavuligerus, and possibly supplies the glyceraldehyde-3-phosphate G3P precursor for CA biosynthesis. The gyl operon is regulated by GylR and is induced by glycerol. To enhance CA production in S. clavuligerus, an extra copy of ccaR expressed from Pgyl the gyl promoter was integrated into the chromosome of S. clavuligerus NRRL 3585. This construct coordinated the transcription of CA biosynthetic pathway genes with expression of the gyl operon. In the transformants carrying the Pgyl-controlled regulatory gene ccaR, CA production was enhanced 3.19-fold in glycerol-enriched batch cultures, relative to the control strain carrying an extra copy of ccaR controlled by its own promoter PccaR. Consistent with enhanced CA production, the transcription levels of ccaR, ceas2 and claR were significantly up-regulated in the transformants containing Pgyl-controlled ccaR.

Keywordsclavulanic acid ccaR glycerol utilization Streptomyces clavuligerus Contributed equally to this work

This article is published with open access at Springerlink.com

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Author: DeKun Guo - YouBao Zhao - KeQian Yang

Source: https://link.springer.com/







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