Effects of L-lactic acid and D,L-lactic acid on viability and osteogenic differentiation of mesenchymal stem cellsReport as inadecuate




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Chinese Science Bulletin

, Volume 58, Issue 20, pp 2404–2411

First Online: 08 May 2013Received: 24 November 2012Accepted: 06 January 2013DOI: 10.1007-s11434-013-5798-y

Cite this article as: He, Y., Wang, W. & Ding, J. Chin. Sci. Bull. 2013 58: 2404. doi:10.1007-s11434-013-5798-y

Abstract

Polylactic acid PLA and other aliphatic polyesters containing the unit of lactic acid are very popular biodegradable materials. While the degradation products, lactic acids, have been worried to bring with negative influence on biocompatibility, the focused experimental studies are less reported. This study is aimed at an in vitro examination of cytotoxicity of both L-lactic acid and D,L-lactic acid. Mesenchymal stem cells MSCs derived from rat bone marrow are employed to test the cytotoxicity of the lactic acids. Considering that the addition of lactic acids not only introduces lactate groups but also alters medium pH and ion strength, these three candidate effects are examined in a decoupled way by setting different comparison groups. The results confirm that the change of medium pH is the predominant factor. It has also been found that D-lactate is more cytotoxic than L-lactate at high concentrations. Yet, either L- or D,L-lactic acids seem acceptable in most of medical applications, because the cytotoxicity is significant only when the concentrations are as high as 20 mmol-L for both of them.

Keywordsbiodegradable polymer biomaterial tissue engineering lactic acid cytotoxicity biocompatibility mesenchymal stem cells This article is published with open access at Springerlink.com

Electronic Supplementary MaterialSupplementary material is available for this article at 10.1007-s11434-013-5798-y and is accessible for authorized users.

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Author: Yao He - WeiRan Wang - JianDong Ding

Source: https://link.springer.com/







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