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wavelet transform, multistage amplifier, capillary electrophoresis, Jansson's deconvolution, overlapping peak separation, signal processing, peak detection

Choi, Sheng Heng

Supervisor and department: Gaudet, Vincent Electrical and Computer Engineering Backhouse, Chris Electrical and Computer Engineering

Examining committee member and department: Bowman, John Mathematics and Statistics Cockburn, Bruce Electrical and Computer Engineering

Department: Electrical and Computer Engineering

Specialization:

Date accepted: 2011-07-08T15:47:54Z

Graduation date: 2011-11

Degree: Master of Science

Degree level: Master's

Abstract: The Applied Miniaturisation Laboratory AML has recently built a laser-induced fluorescent capillary electrophoresis LIF-CE genetic analysis instrument, called the Tricorder Tool Kit TTK. By using a photodiode instead of photomultiplier tubes in the optical detection, the AML has lowered the cost and size compared to commercial LIF-CE products. However, maintaining an adequate signal-to-noise SNR and limit of detection LOD is a challenge.By implementing a multistage amplifier, we increased the bandwidth and voltage swing while maintaining the transimpedance gain compared to the previous design. We also developed signal processing algorithms for post-experiment processing of CE. Using wavelet transform, iterative polynomial baseline fitting, and Jansson-s deconvolution, we improved the SNR, reduced baseline variations, and separated overlapping peaks in CE signals. By improving the electronics and signal processing, we lowered the LOD of the TTK, which is a step towards the realisation of inexpensive point-of-care molecular medical diagnosis instruments.

Language: English

DOI: doi:10.7939-R3Z33Q

Rights: License granted by Sheng Heng Choi sc6@ualberta.ca on 2011-07-07 GMT: Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.





Author: Choi, Sheng Heng

Source: https://era.library.ualberta.ca/



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