Characterization and cloning of a brittle culm mutant bc88 in rice Oryza sativa L.Report as inadecuate




Characterization and cloning of a brittle culm mutant bc88 in rice Oryza sativa L. - Download this document for free, or read online. Document in PDF available to download.

Chinese Science Bulletin

, Volume 58, Issue 24, pp 3000–3006

First Online: 16 May 2013Received: 14 October 2012Accepted: 14 December 2012DOI: 10.1007-s11434-013-5806-2

Cite this article as: Rao, Y., Yang, Y., Xin, D. et al. Chin. Sci. Bull. 2013 58: 3000. doi:10.1007-s11434-013-5806-2

Abstract

This study characterizes a brittle culm bc88 mutant of rice Oryza sativa L. obtained by ethylene methylsulfonate EMS-in-duced mutagenesis of Wuyunjing 7. The bc88 mutant exhibits a diversity of pleiotropic phenotypes, including brittle culm at the whole-plant growth stages, withered leaf tips at the seedling stage, and 18-d delay in heading date at the mature stage. Genetic analysis indicates that the bc88 mutant is controlled by a single recessive nuclear gene. The mutated bc88 gene isolated by map-based cloning contains only one point mutation in the 5th exon relative to its wild-type BC88 LOC Os09g25490 and Os09g0422500, leading to an amino acid change from P to L in bc88 plants. Alignment of the putative protein sequence with its homologs indicates that the mutation is located in the conserved region of the sequence. Detection of the transcription level of BC88 in rice plants shows that the expression level of BC88 is higher in spikes and culms than in leaves, roots, and leaf sheaths. These contribute to understanding of the molecular mechanism of cellulose synthesis. The target gene BC88 can be a useful tool in molecular marker-assisted selection for rice culm trait breeding.

Keywordsrice Oryza sativa L. brittle culm Wuyunjing 7 clone mutation These authors contributed equally to this work.

This article is published with open access at Springerlink.com

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Author: YuChun Rao - YaoLong Yang - DeDong Xin - XiaoJing Li - KaiEn Zhai - BoJun Ma - JianWei Pan - Qian Qian - DaLi Zeng

Source: https://link.springer.com/







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