Insights into food preference in hybrid F1 of Siniperca chuatsi ♀ × Siniperca scherzeri ♂ mandarin fish through transcriptome analysisReport as inadecuate




Insights into food preference in hybrid F1 of Siniperca chuatsi ♀ × Siniperca scherzeri ♂ mandarin fish through transcriptome analysis - Download this document for free, or read online. Document in PDF available to download.

BMC Genomics

, 14:601

Non-human and non-rodent vertebrate genomics

Abstract

BackgroundAs economically relevant traits, feeding behavior and food preference domestication determine production cost and profitability. Although there are intensive research efforts on feeding behavior and food intake, little is known about food preference. Mandarin fish accept only live prey fish and refuse dead prey fish or artificial diets. Very little is currently known about the genes regulating this unique food preference.

ResultsUsing transcriptome sequencing and digital gene expression profiling, we identified 1,986 and 4,526 differentially expressed genes in feeders and nonfeeders of dead prey fish, respectively. Up-regulation of Crbp, Rgr and Rdh8, and down-regulation of Gc expression, consistent with greater visual ability in feeders, could promote positive phototaxis. Altered expressions of period, casein kinase and Rev-erbα might reset circadian phase. Down-regulation of orexigenic and up-regulation of anorexigenic genes in feeders were associated with lower appetite. The mRNA levels of Creb, c-fos, C-EBP, zif268, Bdnf and Syt were dramatically decreased in feeders, which might result in significant deficiency in memory retention of its natural food preference live prey fish. There were roughly 100 times more potential SNPs in feeders than in nonfeeders.

ConclusionsIn summary, differential expression in the genes identified shed new light on why mandarin fish only feed on live prey fish, with pathways regulating retinal photosensitivity, circadian rhythm, appetite control, learning and memory involved. We also found dramatic difference in SNP abundance in feeders vs nonfeeders. These differences together might account for the different food preferences. Elucidating the genes regulating the unique food preference live prey fish in mandarin fish could lead to a better understanding of mechanisms controlling food preference in animals, including mammals.

KeywordsFood preference Live prey fish Mandarin fish Transcriptome sequencing Digital gene expression Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-14-601 contains supplementary material, which is available to authorized users.

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Author: Shan He - Xu-Fang Liang - Jian Sun - Ling Li - Ying Yu - Wei Huang - Chun-Mei Qu - Liang Cao - Xiao-Li Bai - Ya-Xiong Ta

Source: https://link.springer.com/







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