DNMT1 and AIM1 Imprinting in human placenta revealed through a genome-wide screen for allele-specific DNA methylationReport as inadecuate




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BMC Genomics

, 14:685

Human and rodent genomics

Abstract

BackgroundGenomic imprinting is an epigenetically regulated process wherein genes are expressed in a parent-of-origin specific manner. Many imprinted genes were initially identified in mice; some of these were subsequently shown not to be imprinted in humans. Such discrepancy reflects developmental, morphological and physiological differences between mouse and human tissues. This is particularly relevant for the placenta. Study of genomic imprinting thus needs to be carried out in a species and developmental stage-specific manner. We describe here a new strategy to study allele-specific DNA methylation in the human placenta for the discovery of novel imprinted genes.

ResultsUsing this methodology, we confirmed 16 differentially methylated regions DMRs associated with known imprinted genes. We chose 28 genomic regions for further testing and identified two imprinted genes DNMT1 and AIM1. Both genes showed maternal allele-specific methylation and paternal allele-specific transcription. Imprinted expression for AIM1 was conserved in the cynomolgus macaque placenta, but not in other macaque tissues or in the mouse.

ConclusionsOur study indicates that while there are many genomic regions with allele-specific methylation in tissues like the placenta, only a small sub-set of them are associated with allele-specific transcription, suggesting alternative functions for such genomic regions. Nonetheless, novel tissue-specific imprinted genes remain to be discovered in humans. Their identification may help us better understand embryonic and fetal development.

KeywordsGenomic imprinting Placenta Next generation sequencing Differentially Methylated Region DMR DNMT1 AIM1 AbbreviationsCGICpG island

DMRDifferentially methylated region

RRBSReduced representation bisulfite sequencing

MeDIPMethylated DNA immunoprecipitation

DNMT1DNA Methyl Transferase 1

AIM1Absent in Melanoma 1.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-14-685 contains supplementary material, which is available to authorized users.

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Author: Radhika Das - Yew Kok Lee - Ruslan Strogantsev - Shengnan Jin - Yen Ching Lim - Poh Yong Ng - Xueqin Michelle Lin - Kee

Source: https://link.springer.com/



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