The ability of rumen ciliates, Eudiplodinium maggii, Diploplastron affine, and Entodinium caudatum, to use the murein saccharidesReport as inadecuate




The ability of rumen ciliates, Eudiplodinium maggii, Diploplastron affine, and Entodinium caudatum, to use the murein saccharides - Download this document for free, or read online. Document in PDF available to download.

Folia Microbiologica

, Volume 58, Issue 6, pp 463–468

First Online: 28 February 2013Received: 01 February 2012Accepted: 10 February 2013DOI: 10.1007-s12223-013-0231-0

Cite this article as: Belzecki, G., Miltko, R., Kwiatkowska, E. et al. Folia Microbiol 2013 58: 463. doi:10.1007-s12223-013-0231-0

Abstract

Murein polysaccharides may contribute to a considerable part of the dry matter of bacterial cells. Their utilization by protozoa inhabiting the rumen is, however, poorly recognized. The objective of this study was to examine the ability of three species of ciliates, i.e., Eudiplodinium maggii, Diploplastron affine, and Entodinium caudatum of digest, and ferment these saccharides. The cultivation experiments showed that the enrichment of growth medium with bacterial cell wall β-glycans increased the ciliate number p < 0.05. A statistically significant increase p < 0.01 was followed by a continuous decrease p < 0.01 in the percentage of individuals containing β-glycans particles after 4- and 24-h incubation of ciliates with this substrate, respectively. The enzymatic experiments confirmed the ability of the examined protozoa to digest murein. E. caudatum exhibited the highest activity 8.2 unit U-mg protein per min, and E. maggii, the lowest 3.0 U-mg protein per min. The production rates of volatile fatty acids by starved and fed ciliate species were 0.7 and 1.6 E. caudatum pmol-ciliate cell per h, 30.5 and 42.5 E. maggii pmol-ciliate cell per h, and 8.3 and 19.2 D. affine pmol-ciliate cell per h p < 0.05.

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Author: G. Belzecki - R. Miltko - E. Kwiatkowska - T. Michalowski

Source: https://link.springer.com/







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