Cytokine levels as biomarkers of radiation fibrosis in patients treated with breast radiotherapyReport as inadecuate




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Radiation Oncology

, 9:103

Radiation Biology and Molecular Radiation Oncology

Abstract

BackgroundRadiation fibrosis is not easily measurable although clinical scores have been developed for this purpose. Biomarkers present an alternative more objective approach to quantification, and estimation in blood provides accessible samples. We investigated if blood cytokines could be used to measure established fibrosis in patients who have undergone radiotherapy for breast cancer.

MethodsWe studied two cohorts treated by breast-conserving surgery and radiotherapy in the UK START Trial A, one with breast fibrosis cases and one with no or minimal fibrosis controls. Two candidate cytokines, plasma connective tissue growth factor CTGF and serum interleukin-6 IL6 were estimated by ELISA. Comparisons between cases and controls used the t-test or Mann–Whitney test and associations between blood concentration and clinical factors were assessed using the Spearman rank correlation coefficient.

ResultsSeventy patients were included 26 cases, 44 controls. Mean time since radiotherapy was 9.9 years range 8.3-12.0. No statistically significant differences between cases and controls in serum IL6 median IQR 0.84 pg-ml 0.57-1.14, 0.75 pg-ml 0.41-1.43 respectively or plasma CTGF 331.4 pg-ml 234.8-602.9, 334.5 pg-ml 270.0-452.8 were identified. There were no significant associations between blood cytokine concentration and age, fibrosis severity, breast size or time since radiotherapy.

ConclusionsNo significant difference in IL6 or CTGF concentrations was detected between patients with breast fibrosis and controls with minimal or no fibrosis.

KeywordsRadiation fibrosis Breast cancer Biomarker Interleukin-6 Connective tissue growth factor AbbreviationsIL6Interleukin 6

CTGFConnective tissue growth factor

ELISAEnzyme-linked immunosorbent assay

SDStandard deviation

IQRInterquartile range.

Electronic supplementary materialThe online version of this article doi:10.1186-1748-717X-9-103 contains supplementary material, which is available to authorized users.

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Author: Charlotte B Westbury - Joanne Haviland - Sue Davies - Lone Gothard - Bahja Ahmed Abdi - Mark Sydenham - Jo Bowen - Richar

Source: https://link.springer.com/







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