The genome of Romanomermis culicivorax: revealing fundamental changes in the core developmental genetic toolkit in NematodaReport as inadecuate




The genome of Romanomermis culicivorax: revealing fundamental changes in the core developmental genetic toolkit in Nematoda - Download this document for free, or read online. Document in PDF available to download.

BMC Genomics

, 14:923

Comparative and evolutionary genomics

Abstract

BackgroundThe genetics of development in the nematode Caenorhabditis elegans has been described in exquisite detail. The phylum Nematoda has two classes: Chromadorea which includes C. elegans and the Enoplea. While the development of many chromadorean species resembles closely that of C. elegans, enoplean nematodes show markedly different patterns of early cell division and cell fate assignment. Embryogenesis of the enoplean Romanomermis culicivorax has been studied in detail, but the genetic circuitry underpinning development in this species has not been explored.

ResultsWe generated a draft genome for R. culicivorax and compared its gene content with that of C. elegans, a second enoplean, the vertebrate parasite Trichinella spiralis, and a representative arthropod, Tribolium castaneum. This comparison revealed that R. culicivorax has retained components of the conserved ecdysozoan developmental gene toolkit lost in C. elegans. T. spiralis has independently lost even more of this toolkit than has C. elegans. However, the C. elegans toolkit is not simply depauperate, as many novel genes essential for embryogenesis in C. elegans are not found in, or have only extremely divergent homologues in R. culicivorax and T. spiralis. Our data imply fundamental differences in the genetic programmes not only for early cell specification but also others such as vulva formation and sex determination.

ConclusionsDespite the apparent morphological conservatism, major differences in the molecular logic of development have evolved within the phylum Nematoda. R. culicivorax serves as a tractable system to contrast C. elegans and understand how divergent genomic and thus regulatory backgrounds nevertheless generate a conserved phenotype. The R. culicivorax draft genome will promote use of this species as a research model.

KeywordsNematode Genome Evolution Development Caenorhabditis Mermithida Romanomermis Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-14-923 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Philipp H Schiffer - Michael Kroiher - Christopher Kraus - Georgios D Koutsovoulos - Sujai Kumar - Julia I R Camps - Ndif

Source: https://link.springer.com/



DOWNLOAD PDF




Related documents