Designer Self-Assembling Peptide Nanofiber Scaffolds Containing Link Protein N-Terminal Peptide Induce Chondrogenesis of Rabbit Bone Marrow Stem CellsReport as inadecuate




Designer Self-Assembling Peptide Nanofiber Scaffolds Containing Link Protein N-Terminal Peptide Induce Chondrogenesis of Rabbit Bone Marrow Stem Cells - Download this document for free, or read online. Document in PDF available to download.

BioMed Research International - Volume 2014 2014, Article ID 421954, 10 pages -

Research Article

Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China

Department of Gynecology, General Hospital of the Yangtze River Shipping, Wuhan 430022, China

Department of Orthopedics, General Hospital of the Yangtze River Shipping, Wuhan 430022, China

Received 14 April 2014; Revised 30 July 2014; Accepted 30 July 2014; Published 26 August 2014

Academic Editor: Xiuzhi Susan Sun

Copyright © 2014 Baichuan Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Designer self-assembling peptide nanofiber hydrogel scaffolds have been considered as promising biomaterials for tissue engineering because of their excellent biocompatibility and biofunctionality. Our previous studies have shown that a novel designer functionalized self-assembling peptide nanofiber hydrogel scaffold RLN-RADA16, LN-NS containing N-terminal peptide sequence of link protein link N can promote nucleus pulposus cells NPCs adhesion and three-dimensional 3D migration and stimulate biosynthesis of type II collagen and aggrecan by NPCs in vitro. The present study has extended these investigations to determine the effects of this functionalized LN-NS on bone marrow stem cells BMSCs, a potential cell source for NP regeneration. Although the functionalized LN-NS cannot promote BMSCs proliferation, it significantly promotes BMSCs adhesion compared with that of the pure RADA16 hydrogel scaffold. Moreover, the functionalized LN-NS remarkably stimulates biosynthesis and deposition of type II collagen and aggrecan. These data demonstrate that the functionalized peptide nanofiber hydrogel scaffold containing link N peptide as a potential matrix substrate will be very useful in the NP tissue regeneration.





Author: Baichuan Wang, Caixia Sun, Zengwu Shao, Shuhua Yang, Biao Che, Qiang Wu, and Jianxiang Liu

Source: https://www.hindawi.com/



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