Altered expression pattern of miR-29a, miR-29b and the target genes in myeloid leukemiaReport as inadecuate




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Experimental Hematology and Oncology

, 3:17

First Online: 01 July 2014Received: 11 March 2014Accepted: 31 May 2014DOI: 10.1186-2162-3619-3-17

Cite this article as: Xu, L., Xu, Y., Jing, Z. et al. Exp Hematol Oncol 2014 3: 17. doi:10.1186-2162-3619-3-17

Abstract

ObjectivesThe miR-29 family have been demonstrated acting as vital tumor suppressor in multiple cancers as well as regulators in the adaptive immune system. Little is known about their role in leukemogenesis. The purpose of this study is to analyze the expression pattern of miR-29a-29b and its target genes Mcl-1 myeloid cell leukemia sequence 1 and B-cell lymphoma 2 Bcl-2 in myeloid leukemia.

MethodsQuantitative real-time PCR was used for detecting genes expression level in peripheral blood mononuclear cells PBMCs from 10 cases with newly diagnosed, untreated acute myeloid leukemia AML and 14 cases with newly diagnosed, untreated chronic myeloid leukemia CML in chronic phase, and 14 healthy individual HI served as controls. Correlation between the relative expression levels of different genes have been analyzed.

ResultsSignificant lower expression of miR-29a-29b and higher expression level of two potential target genes Bcl-2 and Mcl-1 were found in PBMCs from AML and CML patients compared with HI group. In addtion, miR-29a expression in AML was significantly lower than that in CML. Moreover, negative correlation between miR-29a-29b and its target genes have been found. While, positive correlation between relative expression level of miR-29a and miR-29b or Bcl-2 and Mcl-1 were presented in the total 38 research objects.

ConclusionDown-regulated miR-29a and miR-29b, and accompanying up-regulated Bcl-2 and Mcl-1 are the common feature in myeloid leukemias. These data further support the role for miR-29a-29b dysregulation in myeloid leukemogenesis and the therapeutic promise of regulating miR-29a-29b expression for myeloid leukemia in the future.

AbbreviationsAMLAcute myeloid leukemia

CMLChronic myeloid leukemia

HIHealthy individual Mcl-1, Myeloid cell leukemia sequence 1

Bcl-2B-cell lymphoma 2

RT-PCRReverse transcription–polymerase chain reaction

PBMCsPeripheral blood mononuclear cells

β2-MGβ2-microglobulin

LSCsLeukemia stem cells

CMPCommon myeloid progenitors

CLLChronic lymphocytic leukemia

MCLMantle cell lymphoma

HCCHepatocellular carcinomas.

Electronic supplementary materialThe online version of this article doi:10.1186-2162-3619-3-17 contains supplementary material, which is available to authorized users.

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Author: Ling Xu - Yan Xu - Zhenyi Jing - Xu Wang - Xianfeng Zha - Chengwu Zeng - Shaohua Chen - Lijian Yang - Gengxin Luo - Bo L

Source: https://link.springer.com/







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