Oncogenic viruses associated with vulva cancer in HIV-1 patients in BotswanaReport as inadecuate




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Infectious Agents and Cancer

, 9:28

Clinical oncology

Abstract

BackgroundOncoviruses such as HPV, KSHV, and EBV have been reported in patients with HIV infection and AIDS. How oncovirus-associated cancers rise in AIDS patients has not been fully established. The purpose of our study was to identify the viral agents in vulvar cancer and to assess their contribution to pathogenesis.

MethodWe retrospectively identified a total of 13 vulva tissue samples from HIV-1 positive and 9 vulvar samples from HIV-1 negative patients from the Botswana National Health Laboratory in Gaborone, Botswana, a Southern African Country with a high incidence of HIV. We utilized PCR and IHC to identify HPV, EBV, KSHV, and JC virus in FFPE preserved tissue samples.

ResultsUsing the GP5-GP6 primer set we detected several HPV types in tissue samples. EBV was detected in all of the positive cases 100% and in most of the negative cases 89%. KSHV was detected in 39% of the HIV-1 positive samples and in 11% of the negative samples, and no JC virus was detected in any of the samples.

Using IHC we demonstrated that LANA was expressed in 61% of the positive samples and in 44% of the negative samples. The ubiquitous EBV was more consistently expressed in negative cases 100% than in positive cases 69%. Interestingly, the HPV-16 E6 transcript was detected in 56% of the negative samples compared to 31% of the positive samples. However, the cell cycle protein P21 used as a surrogate marker for HPV was detected in 77% of the positive samples and in 44% of the negative samples, while VEGF signals were similar in both positive 92% and negative samples 89%.

ConclusionOur study, suggests that in Botswana, vulvar squamous cell carcinoma VSCC is associated with oncogenic viruses present in the niche but the contribution and progression may be regulated by HPV and other immunosuppressive infections that include HIV-1.

KeywordsHIV-1 EBV HPV KSHV OSSN Electronic supplementary materialThe online version of this article doi:10.1186-1750-9378-9-28 contains supplementary material, which is available to authorized users.

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Author: Kenneth O Simbiri - Hem C Jha - Mukendi K Kayembe - Carrie Kovarik - Erle S Robertson

Source: https://link.springer.com/







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