Protein Adsorption on Hybrids of Thermoresponsive Polymers and Single-Walled Carbon NanotubesReport as inadecuate




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International Journal of Polymer Science - Volume 2016 2016, Article ID 3539609, 5 pages -

Research Article

Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan

Graduate School of Mathematics and Science Education, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan

Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawadacho, Shinjuku, Tokyo 1620054, Japan

Received 19 February 2016; Revised 7 May 2016; Accepted 17 May 2016

Academic Editor: Dong Wang

Copyright © 2016 Kazuo Umemura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

PolyN-isopropylacrylamide PNIPAAm is one of the most popular thermoresponsive polymers. Adsorption of RecA proteins onto hybrids of PNIPAAm and single-walled carbon nanotubes SWNTs was observed in the presence and absence of DNA molecules. Although RecA molecules were adsorbed efficiently onto the hybrid surfaces at 37°C, even in the absence of DNA molecules, the adsorption of RecA was inhibited at 4°C. These results suggest that the thermoresponsive functions of PNIPAAm were effective, even on the SWNT surfaces, which supports the possibility of developing nanobiodevices using PNIPAAm-SWNT hybrids. However, although RecA is a DNA binding protein, there was no significant difference in the adsorption of RecA onto PNIPAAm-SWNT surfaces with and without DNA molecules. This study provides fundamental information for potential biological applications of PNIPAAm-SWNT hybrids.





Author: Kazuo Umemura, Katsuki Izumi, Yoshikazu Kumashiro, Shusuke Oura, and Teruo Okano

Source: https://www.hindawi.com/



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