Genistein Induces Deleterious Effects during Its Acute Exposure in Swiss MiceReport as inadecuate




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BioMed Research International - Volume 2014 2014, Article ID 619617, 14 pages -

Research Article

Department of Biological Sciences, Indian Institute of Science Education and Research IISER, Bhopal, Madhya Pradesh 462023, India

Toxicology Division, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India

Received 20 February 2014; Revised 14 April 2014; Accepted 15 April 2014; Published 22 May 2014

Academic Editor: Rumiana Koynova

Copyright © 2014 Prabhat Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Genistein is a soy derived isoflavone. It has wide variety of therapeutic effects against certain diseases including cancer. Although toxic effects of genistein have been studied, its effect on the gene expression and the reason behind toxicity have not been identified yet. In the present study, genistein was administered to age and body weight matched Swiss mice at the doses of 125, 250, 500 and 1000 mg-kg. The biomarkers of hepatotoxicity in serum, liver histology, oxidative stress parameters in tissue homogenates, and global gene expression were examined. Elevated alanine aminotransferase ALT, aspartate aminotransferase AST, and alkaline phosphatase ALP levels and degenerated liver tissue were observed in 500, and 1000 mg-kg genistein treated groups. Oxidative stress was significant at these doses as considerable increase in lipid peroxidation LPO and decrease in total glutathione GSH were observed. Gene expression analysis showed 40 differentially expressed genes at twofold change and Differentially expressed genes were corresponding to different biologically relevant pathways including metabolic and oxidative stress pathways. In 500 mg-kg group, Cyp4a14, Sult1e1, Gadd45g, Cidec, Mycs, and so forth genes were upregulated. These results suggested that the higher dose of genistein can produce several undesirable effects by affecting multiple cellular pathways.





Author: Prabhat Singh, Sharad Sharma, and Srikanta Kumar Rath

Source: https://www.hindawi.com/



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