Genome sequencing of four Aureobasidium pullulans varieties: biotechnological potential, stress tolerance, and description of new speciesReport as inadecuate




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BMC Genomics

, 15:549

Eukaryote microbial genomics

Abstract

BackgroundAureobasidium pullulans is a black-yeast-like fungus used for production of the polysaccharide pullulan and the antimycotic aureobasidin A, and as a biocontrol agent in agriculture. It can cause opportunistic human infections, and it inhabits various extreme environments. To promote the understanding of these traits, we performed de-novo genome sequencing of the four varieties of A. pullulans.

ResultsThe 25.43-29.62 Mb genomes of these four varieties of A. pullulans encode between 10266 and 11866 predicted proteins. Their genomes encode most of the enzyme families involved in degradation of plant material and many sugar transporters, and they have genes possibly associated with degradation of plastic and aromatic compounds. Proteins believed to be involved in the synthesis of pullulan and siderophores, but not of aureobasidin A, are predicted. Putative stress-tolerance genes include several aquaporins and aquaglyceroporins, large numbers of alkali-metal cation transporters, genes for the synthesis of compatible solutes and melanin, all of the components of the high-osmolarity glycerol pathway, and bacteriorhodopsin-like proteins. All of these genomes contain a homothallic mating-type locus.

ConclusionsThe differences between these four varieties of A. pullulans are large enough to justify their redefinition as separate species: A. pullulans, A. melanogenum, A. subglaciale and A. namibiae. The redundancy observed in several gene families can be linked to the nutritional versatility of these species and their particular stress tolerance. The availability of the genome sequences of the four Aureobasidium species should improve their biotechnological exploitation and promote our understanding of their stress-tolerance mechanisms, diverse lifestyles, and pathogenic potential.

KeywordsAureobasidium pullulans Dothideomycetes Genome Stress Haloadaptation Halotolerance Polyextremotolerant New species Opportunistic mycosis Pullulan AbbreviationsantiSMASHAntibiotics and secondary metabolites analysis shell

CAFEComputational analysis of gene family evolution

DHNDihydroxynaphthalene

GHGlycoside hydrolases

GTGlucosyltransferase

HOGHigh osmolarity glycerol

MFSMajor facilitator superfamily

MIPMajor intrinsic protein

MAPKMitogen-activated protein kinase

NCBI-NRNational center for biotechnology information non-redundant

PLPolysaccharide lyases.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-15-549 contains supplementary material, which is available to authorized users.

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Author: Cene Gostinčar - Robin A Ohm - Tina Kogej - Silva Sonjak - Martina Turk - Janja Zajc - Polona Zalar - Martin Grube - Hui

Source: https://link.springer.com/







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