Enolase-like protein present on the outer membrane of Pseudomonas aeruginosa binds plasminogenReport as inadecuate




Enolase-like protein present on the outer membrane of Pseudomonas aeruginosa binds plasminogen - Download this document for free, or read online. Document in PDF available to download.

Folia Microbiologica

, Volume 59, Issue 5, pp 391–397

First Online: 27 March 2014Received: 02 January 2013Accepted: 18 February 2014DOI: 10.1007-s12223-014-0311-9

Cite this article as: Ceremuga, I., Seweryn, E., Bednarz-Misa, I. et al. Folia Microbiol 2014 59: 391. doi:10.1007-s12223-014-0311-9

Abstract

Pseudomonas aeruginosa is one of the pathogenic bacteria which utilize binding of the host plasminogen Plg to promote their invasion throughout the host tissues. In the present study, we confirmed that P. aeruginosa exhibits binding affinity for human plasminogen. Furthermore, we showed that the protein detected on the cell wall of P. aeruginosa and binding human plasminogen is an enolase-like protein. The hypothesis that alpha-enolase, a cytoplasmatic glycolytic enzyme, resides also on the cell surface of the bacterium was supported by electron microscopy analysis. The plasminogen-binding activity of bacterial cell wall outer membrane enolase-like protein was examined by immunoblotting assay.

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Author: Ireneusz Ceremuga - Ewa Seweryn - Iwona Bednarz-Misa - Jadwiga Pietkiewicz - Katarzyna Jermakow - Teresa Banaś - Andrzej G

Source: https://link.springer.com/







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