Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminomaReport as inadecuate




Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma - Download this document for free, or read online. Document in PDF available to download.

Biological Research

, 47:52

First Online: 01 October 2014Received: 01 May 2014Accepted: 10 September 2014DOI: 10.1186-0717-6287-47-52

Cite this article as: Shen, CC., Kang, YH., Yu, L.
et al.
biol res 2014 47: 52.
doi:10.1186-0717-6287-47-52

Abstract

BackgroundTestis-expressed sequence 101 TEX101 was found to be highly expressed in testis and involved in acrosome reaction in previous studies.
Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported.
In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.

ResultsImmunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible.
Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia.
Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa.
Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101.

ConclusionsOur data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells.
The lack of TEX101 in seminoma indicated its potential role in tumor progression.
This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

KeywordsImmunohistochemistry TEX101 Seminoma Testis Western blotting Electronic supplementary materialThe online version of this article doi:10.1186-0717-6287-47-52 contains supplementary material, which is available to authorized users.

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Author: Cong-Cong Shen - Yu-Huan Kang - Lin Yu - Dan-Dan Cui - Yi He - Jin-Liang Yang - Lan-Tu Gou

Source: https://link.springer.com/



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