Impact of M36I polymorphism on the interaction of HIV-1 protease with its substrates: insights from molecular dynamicsReport as inadecuate




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BMC Genomics

, 15:S5

First Online: 27 October 2014DOI: 10.1186-1471-2164-15-S7-S5

Cite this article as: Costa, M.G., Benetti-Barbosa, T.G., Desdouits, N. et al. BMC Genomics 2014 15Suppl 7: S5. doi:10.1186-1471-2164-15-S7-S5

Abstract

BackgroundOver the last decades, a vast structural knowledge has been gathered on the HIV-1 protease PR. Noticeably, most of the studies focused the B-subtype, which has the highest prevalence in developed countries. Accordingly, currently available anti-HIV drugs target this subtype, with considerable benefits for the corresponding patients.

However, in developing countries, there is a wide variety of HIV-1 subtypes carrying PR polymorphisms related to reduced drug susceptibility. The non-active site mutation, M36I, is the most frequent polymorphism, and is considered as a non-B subtype marker.

Yet, the structural impact of this substitution on the PR structure and on the interaction with natural substrates remains poorly documented.

ResultsHerein, we used molecular dynamics simulations to investigate the role of this polymorphism on the interaction of PR with six of its natural cleavage-sites substrates.

Free energy analyses by MMPB-SA calculations showed an affinity decrease of M36I-PR for the majority of its substrates. The only exceptions were the RT-RH, with equivalent affinity, and the RH-IN, for which an increased affinity was found. Furthermore, molecular simulations suggest that, unlike other peptides, RH-IN induced larger structural fluctuations in the wild-type enzyme than in the M36I variant.

ConclusionsWith multiple approaches and analyses we identified structural and dynamical determinants associated with the changes found in the binding affinity of the M36I variant. This mutation influences the flexibility of both PR and its complexed substrate. The observed impact of M36I, suggest that combination with other non-B subtype polymorphisms, could lead to major effects on the interaction with the 12 known cleavage sites, which should impact the virion maturation.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-15-S7-S5 contains supplementary material, which is available to authorized users.

Mauricio GS Costa, Técio G Benetti-Barbosa contributed equally to this work.

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Author: Mauricio GS Costa - Técio G Benetti-Barbosa - Nathan Desdouits - Arnaud Blondel - Paulo M Bisch - Pedro G Pascutti - Pa

Source: https://link.springer.com/







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