Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylationReport as inadecuate




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Amino Acids

, Volume 47, Issue 1, pp 199–212

First Online: 02 November 2014Received: 05 August 2014Accepted: 12 October 2014DOI: 10.1007-s00726-014-1857-1

Cite this article as: Pavlyk, I., Rzhepetskyy, Y., Jagielski, A.K. et al. Amino Acids 2015 47: 199. doi:10.1007-s00726-014-1857-1

Abstract

A deficit of exogenous arginine affects growth and viability of numerous cancer cells. Although arginine deprivation-based strategy is currently undergoing clinical trials, molecular mechanisms of tumor cells’ response to arginine deprivation are not yet elucidated. We have examined effects of arginine starvation on cell motility, adhesion and invasiveness as well as on actin cytoskeleton organization of human glioblastoma cells. We observed for the first time that arginine, but not lysine, starvation affected cell morphology, significantly inhibited their motility and invasiveness, and impaired adhesion. No effects on glia cells were observed. Also, arginine deprivation in glioblastoma evoked specific changes in actin assembly, decreased β-actin filament content, and affected its N-terminal arginylation. We suggest that alterations in organization of β-actin resulted from a decrease of its arginylation could be responsible for the observed effects of arginine deprivation on cell invasiveness and migration. Our data indicate that arginine deprivation-based treatment strategies could inhibit, at least transiently, the invasion process of highly malignant brain tumors and may have a potential for combination therapy to extend overall patient survival.

KeywordsAmino acid deprivation Actin cytoskeleton Actin arginylation Cancer Invasiveness Cell migration Electronic supplementary materialThe online version of this article doi:10.1007-s00726-014-1857-1 contains supplementary material, which is available to authorized users.

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Author: Iuliia Pavlyk - Yuriy Rzhepetskyy - Adam K. Jagielski - Jakub Drozak - Anna Wasik - Galyna Pereverzieva - Marta Olchowik -

Source: https://link.springer.com/



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