Wing geometric morphometrics and microsatellite analysis provide similar discrimination of honey bee subspeciesReport as inadecuate




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Apidologie

, Volume 46, Issue 1, pp 49–60

First Online: 26 June 2014Received: 04 November 2013Revised: 06 May 2014Accepted: 04 June 2014DOI: 10.1007-s13592-014-0300-7

Cite this article as: Oleksa, A. & Tofilski, A. Apidologie 2015 46: 49. doi:10.1007-s13592-014-0300-7

Abstract

Identification of honey bee Apis mellifera subspecies is important for their protection. It is also used by queen breeders to maintain some breeding lines. In this study, we compared three methods of subspecies identification based on the following: 17 microsatellite loci, COI-COII mitotypes and geometric morphometrics of forewing venation. The methods were used to classify colonies and workers from a mixed population of A. m. mellifera and A. m. carnica. There was highly significant correlation between results obtained using the three methods. More than three quarters of colonies were classified to the same subspecies by all three methods. The agreement was highest between microsatellites and morphometrics. More than 90 % of colonies were classified to the same subspecies by the two methods. There was also relatively high agreement 75 % between microsatellites and morphometrics when workers were classified as pure subspecies or hybrids. In particular, one pure subspecies was never misclassified as other pure subspecies. The results presented here show that morphometrics can be used for detection of hybrids between A. m. mellifera and A. m. carnica.

Keywordsgeometric morphometrics subspecies discrimination microsatellites mtDNA Bayesian clustering Apis mellifera  phenotype-genotype correlation Manuscript editor: Marina Meixner

Electronic supplementary materialThe online version of this article doi:10.1007-s13592-014-0300-7 contains supplementary material, which is available to authorized users.

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Author: Andrzej Oleksa - Adam Tofilski

Source: https://link.springer.com/







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