Uniparental mitochondrial DNA inheritance is not affected in Ustilago maydis Δatg11 mutants blocked in mitophagyReport as inadecuate




Uniparental mitochondrial DNA inheritance is not affected in Ustilago maydis Δatg11 mutants blocked in mitophagy - Download this document for free, or read online. Document in PDF available to download.

BMC Microbiology

, 15:23

First Online: 06 February 2015Received: 12 November 2014Accepted: 22 January 2015DOI: 10.1186-s12866-015-0358-z

Cite this article as: Wagner-Vogel, G., Lämmer, F., Kämper, J. et al. BMC Microbiol 2015 15: 23. doi:10.1186-s12866-015-0358-z

Abstract

BackgroundMaternal or uniparental inheritance UPI of mitochondria is generally observed in sexual eukaryotes, however, the underlying mechanisms are diverse and largely unknown. Recently, based on the use of mutants blocked in autophagy, it has been demonstrated that autophagy is required for strict maternal inheritance in the nematode Caenorhabditis elegans. Uniparental mitochondrial DNA mtDNA inheritance has been well documented for numerous fungal species, and in particular, has been shown to be genetically governed by the mating-type loci in the isogamous species Cryptococcus neoformans, Phycomyces blakesleeanus and Ustilago maydis. Previously, we have shown that the a2 mating-type locus gene lga2 is decisive for UPI during sexual development of U. maydis. In axenic culture, conditional overexpression of lga2 triggers efficient loss of mtDNA as well as mitophagy. To assess a functional relationship, we have investigated UPI in U. maydis Δatg11 mutants, which are blocked in mitophagy.

ResultsThis study has revealed that Δatg11 mutants are not affected in pathogenic development and this has allowed us to analyse UPI under comparable developmental conditions between mating-compatible wild-type and mutant strain combinations. Explicitly, we have examined two independent strain combinations that gave rise to different efficiencies of UPI. We demonstrate that in both cases UPI is atg11-independent, providing evidence that mitophagy is not critical for UPI in U. maydis, even under conditions of strict UPI.

ConclusionsUntil now, analysis of a role of mitophagy in UPI has not been reported for microbial species. Our study suggests that selective autophagy does not contribute to UPI in U. maydis, but is rather a consequence of selective mtDNA elimination in response to mitochondrial damage.

KeywordsMitophagy Uniparental mitochondrial inheritance mtDNA Ustilago maydis Fungi Atg11 AbbreviationsUPIUniparental inheritance

MTMitochondrial

qPCRQuantitative real-time PCR analysis

WTWild-type

Gaby Wagner-Vogel and Frauke Lämmer contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1186-s12866-015-0358-z contains supplementary material, which is available to authorized users.

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Author: Gaby Wagner-Vogel - Frauke Lämmer - Jörg Kämper - Christoph W Basse

Source: https://link.springer.com/







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