Genome diversification within a clonal population of pandemic Vibrio parahaemolyticus seems to depend on the life circumstances of each individual bacteriaReport as inadecuate




Genome diversification within a clonal population of pandemic Vibrio parahaemolyticus seems to depend on the life circumstances of each individual bacteria - Download this document for free, or read online. Document in PDF available to download.

BMC Genomics

, 16:176

First Online: 13 March 2015Received: 02 July 2014Accepted: 24 February 2015DOI: 10.1186-s12864-015-1385-8

Cite this article as: Loyola, D.E., Navarro, C., Uribe, P. et al. BMC Genomics 2015 16: 176. doi:10.1186-s12864-015-1385-8

Abstract

BackgroundNew strains of Vibrio parahaemolyticus that cause diarrhea in humans by seafood ingestion periodically emerge through continuous evolution in the ocean. Influx and expansion in the Southern Chilean ocean of a highly clonal V. parahaemolyticus serotype O3:K6 population from South East Asia caused one of the largest seafood-related diarrhea outbreaks in the world. Here, genomics analyses of isolates from this rapidly expanding clonal population offered an opportunity to observe the molecular evolutionary changes often obscured in more diverse populations.

ResultsWhole genome sequence comparison of eight independent isolates of this population from mussels or clinical cases from different years was performed. Differences of 1366 to 217,729 bp genome length and 13 to 164 bp single nucleotide variants SNVs were found. Most genomic differences corresponded to the presence of regions unique to only one or two isolates, and were probably acquired by horizontal gene transfer HGT. Some DNA gain was chromosomal but most was in plasmids. One isolate had a large region 8,644 bp missing, which was probably caused by excision of a prophage. Genome innovation by the presence of unique DNA, attributable to HGT from related bacteria, varied greatly among the isolates, with values of 1,366 ten times the number of highest number of SNVs to 217,729 a thousand times more than the number of highest number of SNVs.

ConclusionsThe evolutionary forces SNVs, HGT acting on each isolate of the same population were found to differ to an extent that probably depended on the ecological scenario and life circumstances of each bacterium.

KeywordsVibrio parahaemolyticus Evolution Genome innovation Horizontal gene transfer Single nucleotide variants Electronic supplementary materialThe online version of this article doi:10.1186-s12864-015-1385-8 contains supplementary material, which is available to authorized users.

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Author: David E Loyola - Cristell Navarro - Paulina Uribe - Katherine García - Claudia Mella - Diego Díaz - Natalia Valdes - Jai

Source: https://link.springer.com/







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