Organization and variation analysis of 5S rDNA in gynogenetic offspring of Carassius auratus red var. ♀ × Megalobrama amblycephala ♂Report as inadecuate




Organization and variation analysis of 5S rDNA in gynogenetic offspring of Carassius auratus red var. ♀ × Megalobrama amblycephala ♂ - Download this document for free, or read online. Document in PDF available to download.

BMC Genetics

, 16:26

First Online: 13 March 2015Received: 01 December 2014Accepted: 02 March 2015DOI: 10.1186-s12863-015-0186-z

Cite this article as: Qin, Q., Wang, J., Wang, Y. et al. BMC Genet 2015 16: 26. doi:10.1186-s12863-015-0186-z

Abstract

BackgroundThe offspring with 100 chromosomes abbreviated as GRCC have been obtained in the first generation of Carassius auratus red var. abbreviated as RCC, 2n = 100 ♀ × Megalobrama amblycephala abbreviated as BSB, 2n = 48 ♂, in which the females and unexpected males both are found. Chromosomal and karyotypic analysis has been reported in GRCC which gynogenesis origin has been suggested, but lack genetic evidence.

ResultFluorescence in situ hybridization with species-specific centromere probes directly proves that GRCC possess two sets of RCC-derived chromosomes. Sequence analysis of the coding region 5S and adjacent nontranscribed spacer abbreviated as NTS reveals that three types of 5S rDNA class class I; class II and class III in GRCC are completely inherited from their female parent RCC, and show obvious base variations and insertions-deletions. Fluorescence in situ hybridization with the entire 5S rDNA probe reveals obvious chromosomal loci class I and class II variation in GRCC.

ConclusionsThis paper provides directly genetic evidence that GRCC is gynogenesis origin. In addition, our result is also reveals that distant hybridization inducing gynogenesis can lead to sequence and partial chromosomal loci of 5S rDNA gene obvious variation.

KeywordsDistant hybridization Gynogenesis 5S rDNA FISH QinBo Qin and Juan Wang contributed equally to this work.

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Author: QinBo Qin - Juan Wang - YuDe Wang - Yun Liu - ShaoJun Liu

Source: https://link.springer.com/







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