Role of AcsR in expression of the acetyl-CoA synthetase gene in Vibrio vulnificusReport as inadecuate




Role of AcsR in expression of the acetyl-CoA synthetase gene in Vibrio vulnificus - Download this document for free, or read online. Document in PDF available to download.

BMC Microbiology

, 15:86

First Online: 12 April 2015Received: 18 July 2014Accepted: 25 March 2015DOI: 10.1186-s12866-015-0418-4

Cite this article as: Kim, M.J., Kim, J., Lee, H.Y. et al. BMC Microbiol 2015 15: 86. doi:10.1186-s12866-015-0418-4

Abstract

BackgroundVarS-VarA is one of the global factors regulating diverse aspects of the metabolism and virulence of bacteria including pathogenic Vibrio spp. An experiment to identify the VarS-VarA-regulon in V. vulnificus revealed that a putative LuxR-type transcriptional regulator was down-regulated in ΔvarA mutant. To investigate the roles of this regulatory cascade, the target gene regulated by a LuxR-regulator was identified and its expression was characterized.

ResultsTranscriptomic analysis of the mutant deficient in this LuxR-type regulator showed that the acsA gene encoding acetyl-CoA synthetase was down-regulated. Thus, this regulator was named AcsR for -regulator of acetyl-CoA synthetase-. A putative histidine kinase gene, acsS, was located five ORFs downstream of the acsR gene. Expression of an acsA::luxAB transcriptional fusion was decreased in both ΔacsR and ΔacsS mutants. Similar to a ΔacsA mutant, strains carrying deletions either in acsR or acsS grew slowly than wild type in a minimal medium with acetate as a sole carbon source. Growth defect of the ΔacsR strain in acetate-minimal medium was restored by complementation. To investigate if AcsR directly regulates acsA expression, in vitro-gel shift assays were performed using the recombinant AcsR and the regulatory region of the acsA gene, showing that AcsR specifically bound the upstream region of the acsA ORF.

ConclusionThis study indicates that the VarS-VarA system plays a role in V. vulnificus metabolism via regulating AcsR, which in turn controls acetate metabolism by activating the transcription of the acetyl-CoA synthetase gene.

KeywordsVibrio vulnificus Acetate metabolism Acetyl-CoA synthetase AcsR VarS-VarA AbbreviationsAcsAcetyl-CoA synthetase

AckAcetate kinase

PtaPhosphtransacetylase

rAcsRRecombinant AcsR

LBLuria-Bertani

RLURelative light unit

COGCLUSTER of orthologous groups

MASHMannose-sensitive hemagglutinin

Electronic supplementary materialThe online version of this article doi:10.1186-s12866-015-0418-4 contains supplementary material, which is available to authorized users.

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Author: Min Jung Kim - Juri Kim - Hye Yeon Lee - Hyeon Jin Noh - Kyu-Ho Lee - Soon-Jung Park

Source: https://link.springer.com/



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