Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thalianaReport as inadecuate




Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana - Download this document for free, or read online. Document in PDF available to download.

BMC Biotechnology

, 15:26

First Online: 12 April 2015Received: 21 October 2014Accepted: 03 March 2015DOI: 10.1186-s12896-015-0138-8

Cite this article as: Bakar, F.A., Yeo, C.C. & Harikrishna, J.A. BMC Biotechnol 2015 15: 26. doi:10.1186-s12896-015-0138-8

Abstract

BackgroundBacterial toxin-antitoxin systems usually comprise of a pair of genes encoding a stable toxin and its cognate labile antitoxin and are located in the chromosome or in plasmids of several bacterial species. Chromosomally-encoded toxin-antitoxin systems are involved in bacterial stress responses and activation of the toxins usually leads to cell death or dormancy. Overexpression of the chromosomally-encoded YoeB toxin from the yefM-yoeB toxin-antitoxin locus of the Gram-positive bacterium Streptococcus pneumoniae has been shown to cause cell death in S. pneumoniae as well as E. coli.

ResultsInduction of a YoeB-GFP fusion protein using a 17-β-estradiol-inducible plant expression system in Arabidopsis thaliana Col 0, was lethal in all T2 progeny. Examination of plants by fluorescent confocal microscopy showed GFP fluorescence in all parts of the leaves at 24 hours after 17-β-estradiol induction, continuing up to plant death. Quantitative RT-PCR analysis revealed that the expression of the yoeB toxin gene peaked at 3 days after induction with 17-β-estradiol, coinciding with the onset of visible effects on the plants. Moreover, we detected DNA laddering in the transgenic plants at 24 hours after yoeB induction, indicative of apoptosis.

ConclusionsExpression of the YoeB toxin from Streptococcus pneumoniae is lethal in Arabidopsis. We believe this is the first report of a toxin from a bacterial toxin-antitoxin system functioning in plants. The results presented here mark an important milestone towards the development of a cell ablation based bio-containment strategy, which may be useful for functional studies and for the control of spread of transgenic plants.

KeywordsToxin-Antitoxin system Bacteria Arabidopsis thaliana 17-β-estradiol Expression AbbreviationsCTABCetyltrimethylammonium bromide

GFPGreen fluorescent protein

SpnStreptococcus pneumoniae

TAToxin-antitoxin

An erratum to this article can be found at http:-dx.doi.org-10.1186-s12896-015-0220-2.

An erratum to this article is available at http:-dx.doi.org-10.1186-s12896-015-0220-2.

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Author: Fauziah Abu Bakar - Chew Chieng Yeo - Jennifer Ann Harikrishna

Source: https://link.springer.com/



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