A three-miRNA signature as promising non-invasive diagnostic marker for gastric cancerReport as inadecuate




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Molecular Cancer

, 14:202

First Online: 25 November 2015Received: 06 July 2015Accepted: 18 November 2015DOI: 10.1186-s12943-015-0473-3

Cite this article as: Shin, V.Y., Ng, E.K.O., Chan, V.W. et al. Mol Cancer 2015 14: 202. doi:10.1186-s12943-015-0473-3

Abstract

BackgroundDespite the declining incidence of gastric cancer, mortality rate remains high due to late presentation. We aimed to evaluate the sensitivity of miRNA as a diagnostic marker for gastric cancer in the circulation.

MethodsPlasma samples from 3 independent groups comprise 123 gastric cancer patients and 111 healthy controls for miRNA profiling from microarray screening.

ResultsMicroarray data showed that 25 miRNAs were upregulated in gastric cancer patients and 6 highly expressed miRNAs miR-18a, miR-140-5p, miR-199a-3p, miR-627, miR-629 and miR-652 were selected for validation. In an independent validation set, levels of miR-627, miR-629 and miR-652 were significantly higher in gastric cancer patients than healthy controls P <0.0001. An algorithm with improved sensitivity and specificity as gastric cancer classifier was adopted and validated in another random set of 15 plasma samples. Results showed that combination of 3 miRNAs obtained the highest area under curve, with a cut-off at 0.373, with a sensitivity of 86.7 % and a specificity of 85.5 %.

ConclusionThis study revealed a three-miRNA signature as a promising classifier for gastric cancer, and greatly enhances the feasibility of circulating miRNAs as non-invasive diagnostic marker for this disease.

KeywordsCirculating microRNA Gastric cancer Diagnostic marker Electronic supplementary materialThe online version of this article doi:10.1186-s12943-015-0473-3 contains supplementary material, which is available to authorized users.

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Author: Vivian Yvonne Shin - Enders K. O. Ng - Vivian W. Chan - Ava Kwong - Kent-Man Chu

Source: https://link.springer.com/







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