piRNA-like small RNAs mark extended 3’UTRs present in germ and somatic cellsReport as inadecuate

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BMC Genomics

, 16:462

First Online: 16 June 2015Received: 23 December 2014Accepted: 29 May 2015DOI: 10.1186-s12864-015-1662-6

Cite this article as: Yamtich, J., Heo, SJ., Dhahbi, J. et al. BMC Genomics 2015 16: 462. doi:10.1186-s12864-015-1662-6


BackgroundPiwi-interacting RNAs piRNAs are a class of small RNAs; distinct types of piRNAs are expressed in the mammalian testis at different stages of development. The function of piRNAs expressed in the adult testis is not well established. We conducted a detailed characterization of piRNAs aligning at or near the 3’ UTRs of protein-coding genes in a deep dataset of small RNAs from adult mouse testis.

ResultsWe identified 2710 piRNA clusters associated with 3’ UTRs, including 1600 that overlapped genes not previously associated with piRNAs. 35 % of the clusters extend beyond the annotated transcript; we find that these clusters correspond to, and are likely derived from, novel polyadenylated mRNA isoforms that contain previously unannotated extended 3’UTRs. Extended 3’ UTRs, and small RNAs derived from them, are also present in somatic tissues; a subset of these somatic 3’UTR small RNA clusters are absent in mice lacking MIWI2, indicating a role for MIWI2 in the metabolism of somatic small RNAs.

ConclusionsThe finding that piRNAs are processed from extended 3’ UTRs suggests a role for piRNAs in the remodeling of 3’ UTRs. The presence of both clusters and extended 3’UTRs in somatic cells, with evidence for involvement of MIWI2, indicates that this pathway is more broadly distributed than currently appreciated.

KeywordspiRNA 3’ UTR Somatic MIWI2 Electronic supplementary materialThe online version of this article doi:10.1186-s12864-015-1662-6 contains supplementary material, which is available to authorized users.

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Author: Jennifer Yamtich - Seok-Jin Heo - Joseph Dhahbi - David IK Martin - Dario Boffelli

Source: https://link.springer.com/

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